Autoclaving: Difference between revisions

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The autoclave was re-invented for medical and scientific use by Charles Chamberland in 1879. Chamberland was a microbiologist who worked with Louis Pasteur<ref name="Qor" />.

==Operation of an Autoclave==

Items to be autoclaved are subjected to gradual temperature increases under high pressure until 121 °C is reached and then steamed for around 15–20 minutes<ref name="Qor" />. The autoclave allows steam to flow around items in the chamber. The length of time and temperature necessary for sterilization depend on the items to be sterilized and whether they are wrapped or left directly exposed to the steam,ref name="Qor" />. Items should be separated to allow the steam to penetrate the load evenly. The steam can reach in small crevices and can kill all bacteria, viruses and bacterial spores<ref name="Qor" />.

Autoclaves can be used to eliminate microorganismsand for hydrothermal synthesis<ref name ="Qor" />. [[Autoclaving]] is a very dependable method for the sterilization and decontamination of laboratory glassware, medical instruments and certain types of [[Healthcare Waste]], reagents, and other media. Autoclaves can inactivate fungi, bacteria, spores, viruses and other microorganisms on surgical instruments such as scalpels, forceps, scissors and other metal items,ref name="Qor" />.

==References==

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 The autoclave was re-invented for medical and scientific use by Charles Chamberland in 1879. Chamberland was a microbiologist who worked with Louis Pasteur<ref name="Qor" />.
+==Operation of an Autoclave==
+Items to be autoclaved are subjected to gradual temperature increases under high pressure until 121 °C is reached and then steamed for around 15–20 minutes<ref name="Qor" />. The autoclave allows steam to flow around items in the chamber. The length of time and temperature necessary for sterilization depend on the items to be sterilized and whether they are wrapped or left directly exposed to the steam,ref name="Qor" />. Items should be separated to allow the steam to penetrate the load evenly. The steam can reach in small crevices and can kill all bacteria, viruses and bacterial spores<ref name="Qor" />.
+Autoclaves can be used to eliminate microorganismsand for hydrothermal synthesis<ref name ="Qor" />. [[Autoclaving]] is a very dependable method for the sterilization and decontamination of laboratory glassware, medical instruments and certain types of [[Healthcare Waste]], reagents, and other media. Autoclaves can inactivate fungi, bacteria, spores, viruses and other microorganisms on surgical instruments such as scalpels, forceps, scissors and other metal items,ref name="Qor" />.
 ==References==
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